Tfeb s142
Web2 Feb 2024 · A Representative Western blots showed the levels of phosphorylated (p-) TFEB (S142) and the lysosome marker LAMP1 in the hippocampi of mice from each group. B , C … WebTFEB has also been shown to be activated in a nutrient-dependent manner by p42 MAP kinase (Erk2). TFEB is phosphorylated at Ser142 by Erk2 in response to nutrient …
Tfeb s142
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Web8 Sep 2024 · tfeb在s142的磷酸化还由erk2介导。 图4 tfeb 结构域结构中的磷酸化位点及其调节作用. 最近研究还发现stub1通过靶向磷酸化的tfeb(s142和s211)进行泛素介导的蛋白酶 … WebTFEB is sequestered in the cytosol by inhibitory phosphorylation at Ser142 (P-TFEB S142 ) 25 . In line with earlier observations, 6 h and 24 h S− and PE treatment sig- nificantly …
Web17 Aug 2024 · In particular, TFEB S142A showed impaired phosphorylation of S142 and S138; TFEB S138A showed impaired phosphorylation of S138, but normal S142 … WebPolyclonal Antibody for studying TFEB (Ser122) phosphate. Cited in 6 publications. Validated for Western Blotting, Immunoprecipitation. Highly specific and rigorously validated in …
HeLa TFEB-GFP cells were seeded in 96-well plates and incubated for 24 h. After incubation cells were treated as described above, rinsed with PBS once, fixed for 10 min with 4% paraformaldehyde and stained with DAPI. For the acquisition of the images, at least ten image fields were acquired per well of the 96 … See more Cells were cultured in the following media: HeLa (ATCC) in MEM (Cat# ECB2071L, Euroclone); HEK293T (ATCC) in DMEM high glucose (Cat# ECM0728L, Euroclone); ARPE19 (ATCC) in DMEM-F12 (Cat# 11320–033, Thermo … See more Reagents used in this study were obtained from the following sources: Antibodies to Phospho-p70 S6 Kinase (Thr389) (1A5) (Cat# 9206) (1:1000), p70 S6 Kinase (Cat# 9202) (1:1000), 4E-BP1 (Cat# 9644) (1:1000), Phospho-4E … See more The plasmid-encoding ΔNLS-TFEB-GFP was a kind gift of Shawn Ferguson (Yale School of Medicine, CT, USA). TFEB S138A-GFP was a … See more For experiments involving amino acid starvation, cell culture plates were rinsed twice with PBS and incubated in amino acid-free RPMI (Cat# R9010-01, USBiological) supplemented with 10% dialyzed FBS for 60 … See more
Web10 Nov 2024 · Upon cellular stress signals, such as nutrient deprivation, inhibition of the amino acid sensing mTOR complex 1 (mTORC1) results in accumulation of TFEB dephosphorylated at S142 and S211, which dissociates from 14-3-3 proteins, translocates into the nucleus, and activates its transcriptional program (Martina et al., 2012; Roczniak …
Web1 Apr 2024 · TFEB subcellular localization is mainly regulated by phosphorylation of its specific serine residues and mutation of these serine residues 142/211 (S142/S211) into … can a bankruptcy stop a wage garnishmentWeb6 May 2024 · For example, TFEB activity can be suppressed by extracellular signal-regulated kinase 2 (ERK2) through the phosphorylation of TFEB at S142 . Interestingly, ERK … fishbonenWebThe activity of TFEB is tightly linked to nutrient availability via protein phosphorylation. In the presence of nutrients TFEB is phosphorylated by mTOR on S142 and S211 serine … fishbone offroad bed storage rackWeb(ABE1971) Anti-phospho TFEB (Ser142) Antibody Sign in or register to save this reagent to your favourites Supplier MilliporeSigma Host Rabbit Type Primary Clonality Polyclonal … fishbonen one pieceWeb1 Jan 2015 · Phosphoproteomic studies identified at least 10 different phosphorylation sites on the TFEB protein, while mutation analysis suggested that only phosphorylation at Ser211 and Ser142 is critical for TFEB subcellular localization ( Martina et al., 2012, Roczniak-Ferguson et al., 2012, Settembre et al., 2012 ). fishbone offroad bumperWebUpon nutrient-refeeding, TFEB and TFE3 are exported back to the cytoplasm, although little is known about how nuclear export is regulated. ... Here we … fish bone needle stick injuryWebBlot was analyzed by staining with the indicated antibodies, phospho-S6 (S235/S236) is the indicator of increased mTORC1 activity, NPC1 is the indicator of TFEB transcriptional activity. g Densitometry for phospho TFEB S142 and phospho TFEB S211 was performed using ImageJ and normalized to total TFEB-GFP ( n = 3 biological replicates per … fishbone offroad decal