Phospho-upf1
WebSep 1, 2006 · Since the phosphorylation of Upf1 by SMG-1 is a critical rate-limiting step for NMD, the decrease in phospho-Upf1 suggested that cellular NMD is suppressed. FIG. 2 Knockdown of Upf1 or SMG-1 by synthetic siRNA inhibits NMD, resulting in the accumulation of PTC-containing COL α2 (VI) mRNA and collagen VI protein. (A) Synthetic siRNA … WebSMG-1-mediated Upf1 phosphorylation takes place in the decay inducing complex (DECID), which contains a ribosome, release factors, Upf1, SMG-1, an exon junction complex (EJC) …
Phospho-upf1
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WebSep 29, 2011 · SMG-1-mediated Upf1 phosphorylation takes place in the decay inducing complex (DECID), which contains a ribosome, release factors, Upf1, SMG-1, an exon junction complex (EJC) and a PTC-mRNA. However, the significance and the consequence of Upf1 phosphorylation remain to be clarified. WebDec 14, 2024 · While preclearing each lysate, prepare the antibody−Dynabeads Protein A (see Note 10) by adding 5–10 μg of anti-phospho-UPF1 (Ser1116) antibody or, as a negative control, rabbit IgG (see Note 11) to 50 μl of washed Dynabeads Protein A from Subheading 3.3, step 1 in 300 μl of Hypotonic Gentle Lysis Buffer containing 150 mM NaCl (see Note …
WebUPF1 is an important factor for the RNA quality control system and the regulation of physiological gene expression, and contributes to DNA replication, DNA repair, telomere … WebAnti-phospho-Upf1 (Ser1127), Cat. No. 07-1016, is a highly specific rabbit polyclonal antibody that targets Regulator of nonsense transcripts 1 (RENT1) phosphorylated at …
WebPan Phospho-Tyrosine (2) Phospho Y705 (1) MonoMethyl K4 (1) Phospho S9 (1) DiMethyl K9 (1) DiMethyl K4 (2) Unconjugated (11) Phospho Y15 (1) TriMethyl K36 (2) Phospho S15 (1) ... UPF1 UPF1; DNMT1 DNMT1; FURIN FURIN; ACLY ACLY; ALDH1A1 ALDH1A1; PROM1 PROM1; MCAM MCAM; CD62P CD62P; P-SELECTIN P-SELECTIN; CALR CALR; CLTC … WebJul 10, 2014 · The phospho-independent UPF1 interaction motif of SMG6 localized to a stretch that is proximal to the TPR domain and distinct from the EBMs located within the first 160 residues. (D) GST pull-down ...
Webanti–phospho-S/T-Q antibody–detected band comigrating with Upf1 in both the lysate and anti-Upf1 immunoprecipitate (Fig. 1E). This result confirms that disruption of Smg1 expression in Smg1gt/gt embryos reduces the phosphorylation levels of Upf1. Developmental Defects in Smg1gt/gt Embryos. Examination of
WebDec 10, 2024 · Fig. 3: Correlation of NMD efficiency with NMD regulator SMG1 and phosphorylation of UPF1. Simultaneous detection of fluorescence NMD reporter and SMG1, or phospho-UPF1 (PUPF1). a, d... philosophy bath washWebAnti-phospho-Upf1 (Ser1127), Cat. No. 07-1016, is a highly specific rabbit polyclonal antibody that targets Regulator of nonsense transcripts 1 (RENT1) phosphorylated at Ser1127 and has been tested in More>> Anti-phospho-Upf1 (Ser1127) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available … t shirt getting to know youWebMay 29, 2010 · Compared with WT, GT tMEFs showed reduced levels of an anti–phospho-S/T-Q antibody–detected band comigrating with Upf1 in both the lysate and anti-Upf1 immunoprecipitate (Fig. 1E). This result confirms that disruption of Smg1 expression in Smg1 gt/gt embryos reduces the phosphorylation levels of Upf1. t shirt gerwyn priceWebFeb 4, 2016 · UPF1 is a 130-kDa RNA helicase composed of two recombinase A (RecA)-like domains at its C terminus and an N-terminal regulatory domain 8, 9. In its closed conformation, an N-terminal... t shirt geometricWebJul 3, 2024 · UPF1 overexpression protects neurons from R-DPR toxicity Consistent with the notion that R-DPRs cause neurodegeneration in part through inhibiting NMD, recent studies have shown that... t shirt gentsWebDownload scientific diagram Replication stress results in increased phosphorylation of UPF1 but not HBP/SLBP on PIKK substrate motifs. Asynchronous HeLa cells were incubated with or without 2 mM ... t-shirt germanyWebApr 18, 2008 · Upf1 phosphorylation triggers translational repression during nonsense-mediated mRNA decay In mammalian cells, nonsense-mediated mRNA decay (NMD) … t shirt germany