Phis-sumo vector

Webb28 sep. 2024 · Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV … WebbOur SUMO-tag expression systems maximize the yield of soluble, functional proteins in E. coli. SUMO functions as both a chaperonin and initiator of protein folding, improving the …

A M55V Polymorphism in a Novel SUMO Gene (SUMO-4) …

Webbsumo标签蛋白是一种小分子泛素相关修饰蛋白,是存在于真核生物中高度保守的参与蛋白质小泛素化相关修饰的一类大蛋白。 与GST、MBP或NusA相比,SUMO不仅可以作为重组 … WebbpET SUMO (linearized) Linearized bacterial vector with 3'-T overhangs for TA cloning and inducible expression of an N-terminally 6xHis- and SUMO-tagged protein. Sequence … simply life metroplaza https://naughtiandnyce.com

Pseudovector - Wikipedia

Webb635690: pCMV-HA Vector Set. 635689. pCMV-Myc Vector Set. Each. USD $534.00. This vector set allows for cloning and expression of your gene of interest fused to a Myc tag at either the N- or C-terminus. Notice to purchaser. Our … WebbExpresso T7 SUMO Cloning Kit Container Store at -20°C Concentration Volume pETite N-His SUMO Kan Vector DNA (5 reactions) 12.5 ng/ l 15 l SUMO Positive Control C Insert … WebbpGADT7 AD is a yeast two-hybrid expression vector that is designed to express a fusion protein between the GAL4 activation domain (AD; amino acids 768–881) and a protein of interest. The fusion protein is expressed at high levels from the full-length ADH1 promoter. The fusion protein also contains a hemagglutinin (HA) epitope tag. simply life mortgages

pET-N-His-PreScission-SUMO(原核表达质粒)(D2918-1μg)

Category:Champion™ pET SUMO Expression System

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Phis-sumo vector

pSUMO大肠杆菌表达载体说明 - 豆丁网

WebbWe have a modified pETM11 vector for the expression of SUMO3 fusion proteins available. This vector contains an optimized synthetic human sumo3 gene with appropiate …

Phis-sumo vector

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WebbNovelMechanismofHeminCapturebyHbp2,the Hemoglobin-bindingHemophorefromListeria monocytogenes* … Webbsmall size of the pRham N-His SUMO Kan Vector (2.6 kb) facilitates cloning of large inserts and performing DNA manipulations, such as site- directed mutagenesis. The pRham N …

WebbThe IsdH expression vector used in this study was a modi- fied version of the pRM208 plasmid, which contained the second and third NEAT domains and the intervening … WebbTumor-Linked Macrophages Promote HCC Development by Mediating the CCAT1/Let-7b/HMGA2 Signaling Pathway

Webb27 feb. 2024 · However, removing SUMO is a labor- and time-consuming process. To enhance this system, here we show the construction of a bicistronic vector that allows … WebbVector type Bacterial Expression Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number Low Copy …

Webb26 maj 2024 · 载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically …

WebbChampion™ pET SUMO 表达系统作为完整的系统提供。Champion™ pET SUMO TA Cloning™ 盒含有线性化 Champion™ pET SUMO 载体、无菌水、dNTP、10X PCR 缓冲 … simplylife personal loanWebb22 okt. 2009 · Plasmid constructs. A human RARA (hRARA) cDNA from LRARaSN was subcloned into the pFLAG-CMV2 vector (Sigma-Aldrich Co., St. Louis, MO) using EcoRI … simply life organicsWebbyeast 2-hybrid vectors (sundquist lab) pGADT7 and pGBKT7. Ligation Independent Cloning (LIC) vectors from Mark Connelly: pMCSG10, pMCSG7, pMCSG17-20, pMCSG19, … raytheon product catalogWebbThe pSUMO vector is a low copy plasmid with kanamycin selection. ( b ) The DNA sequence encoding the SUMO tag. The restriction digestion site of enzyme BsaI is … simply life photographyWebbencoding amino acids 1-96 of SUMO-1 (lacking glycine 97 creating a SUMO-1 that is not susceptible to proteolytic removal by isopeptidases) was fused in frame to the amino … simply life personal loanWebbSUMO-4V constructs were made to express active recombinant proteins ending in the terminal di-glycine residues (Fig. 1, additional C-terminal residues are cleaved normally in … raytheon produkteWebbWe have a modified pETM11 vector for the expression of SUMO3 fusion proteins available. This vector contains an optimized synthetic human sumo3 gene with appropiate restriction sites at the 3′-end of the sumo3 gene for the insertion of any gene of interest.. The small human SUMO3 protein (81 aa, 9.2 kDa) can be used as an N-terminal fusion partner for … raytheon product line