WebHepaRG™ cells are metabolically competent cells that express Phase I and II metabolic enzymes and differentiate into mature hepatocyte-like cells, making them ideal for toxicity testing. Webmicronucleus formation and a stable karyotype be used. 12. Human peripheral blood lymphocytes should be obtained from young (approximately 18-35 years of age), healthy, …
Performance of HepaRG and HepG2 cells in the high-throughput ...
Web1 okt. 2024 · The HepaRG human hepatic progenitor cell line was selected for the micronucleus assay because its cytochrome P450 enzyme activities have been well documented in the literature and have been found to closely reflect those in primary human hepatocytes (Guillouzo et al., 2007; Turpeinen et al., 2009 ). Web1 The International Transporter Consortium (2010) Membrane transporters in drug development. Nat Rev Drug Disc 9(3); 215–236 2 Wakasugi H et al. (1998) Effect of clarithromycin on renal excretion of digoxin: Interaction with P-glycoprotein. Clin Pharmacol Ther 64(1); 123 -128 3 FDA Guidance for Industry – In Vitro Drug Interaction Studies - … college humor besties with testies
HepaRG™ CometChip® Micronucleus Assay as a New Alternative …
Web1 apr. 2024 · However, currently standardised in vitro methods for genotoxicity testing using mammalian cells (HPRT and TK assays, micronucleus assay) are tedious and not suitable for high-throughput testing. One of the promising markers of DNA damage response for genotoxicity assessment seems to be the phosphorylation of the histone variant H2AX [ 5 ]. WebThe aim of this study was to evaluate the suitability of differentiated human hepatoma HepaRG cells as an in vitro model system for the detection of DNA damage induced by … Web16 nov. 2024 · The HepaRG MN assay was 100% specific and 93% accurate in detecting genotoxic potential of the 28 compounds. Quantitative comparison of the MN concentration-response data using benchmark dose analysis showed that most of the … collegehumor bathroom numbers