Heparg differentiation

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August undefined, 2024

Web12 feb. 2024 · HepaRG is a bipotent stem cell line that can be differentiated towards hepatocyte-like and biliary-like cells. The entire cultivation process requires 1 month and … WebOur results indicated that the enhancement of hepatic functions in 3D-cultured HepaRG cells was induced by selective hepatocyte differentiation and accelerated hepatocyte …

The HepaRG cell line: biological properties and relevance as a

WebHere, we present a novel hepatic maturation medium (HMM) for the differentiation of HepaRG, which is based on a cocktail of soluble molecules that mimick the in vivo … WebDifferentiation of HepaRG-hepatoblasts into cholangiocytes. (A) Diagram of both protocols for HepaRG cell differentiation from the progenitor stage into hepatocytes or cholangiocytes. Scale... fip in humans

Comparison of HepaRG cells following growth in proliferative and ...

Web28 mrt. 2007 · Whereas HNF1α and HNF3β were expressed in HepaRG cells at the progenitor stage as in oval cells, HNF4α, known to be necessary during the final step of hepatocyte differentiation, 38, 39 was up-regulated during the differentiation process. 40 However, the molecular mechanisms that at an early stage determine if bipotent … Web1 aug. 2024 · In contrast to many hepatoma cell lines, differentiated HepaRG cells exhibit specific liver functions resembling those of adult primary human hepatocytes, including abundant CYP450 enzymes, such as CYP1A2, CYP2B6, CYP2E1, and CYP3A4, phase II enzymes, drug transporters, and ligand-activated nuclear receptors [3], [4], [5], [6], [7]. WebHepaRG cells have superior expression levels of key uptake and efflux transporters compared to other cell lines and expression levels closely resembling those of human hepatocytes (Figures 5 and 6). They also … essential oils for heart energy

Fast Differentiation of HepaRG Cells Allowing Hepatitis B and Delta ...

A DMSO-free hepatocyte maturation medium accelerates hepatic ...

Web一站式科研服务平台. 学术工具. 文档翻译; 收录引证; 论文查重; 文档转换 WebA) Compared to the 2D plated cells the proliferation of the low-density progenitor cells in the hydrogels was remarkably slower. B) At high-density cultures, the differentiated cells did not... essential oils for heart failureWebHepaRG® Differentiation Medium Supplement with antibiotics. For the use of HepaRG® cell line, as supplement for differentiation medium, according to BIOPREDIC … essential oils for heartworm prevention

"Web11 nov. 2024 · HepaRG cells show high plasticity in 2D culture, being able to differentiate into biliary and hepatocyte-like cells after exposure to DMSO (Aninat et al. 2006). Our … " - Heparg differentiation

Heparg differentiation

The importance of transporters and cell polarization for the …

WebHepaRG™ cells exhibit many characteristics of primary human hepatocytes and are essentially a single donor. These features allow users to obtain physiological relevant results for metabolism-based drug-drug interactions without the concern of donor variability and limited lot sizes that come with relying on donor tissue.

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Web27 okt. 2024 · Data Availability Statement. The raw data can be assessed from ENA: In vivo liver: PRJEB35350/ERP118386, PHH: PRJEB23590/ERP105351, iPSC-HLC: PRJEB23620/ERP105382, 3D liver microtissues: PRJEB24482/ERP106310, HepG2: PRJEB24466/ERP106294 and PRJEB24464/ERP106292, HepaRG 3D: … Web13 aug. 2024 · Differentiated HepaRG (dHepaRG) are considered the best surrogate model to primary human hepatocytes (PHH) and are susceptible to several hepatotropic …

Web4 feb. 2024 · The in vitro generation of terminally differentiated hepatocytes is an unmet need. We investigated the contribution of oxygen concentration to differentiation in human liver cell lines HepaRG and C3A. HepaRG cells were cultured under hypoxia (5%O2), normoxia (21%O2) or hyperoxia (40%O2). Cultures were analysed for hepatic functions, … Web26 mei 2024 · Further, NRF2 inhibition drives the trans-differentiation of HepaRG cells, a human liver-derived cell line that shows phenotypical markers of hepatocyte-like and cholangiocyte-like cells 22.

Web12 feb. 2024 · HepaRG is a bipotent stem cell line that can be differentiated towards hepatocyte-like and biliary-like cells. The entire cultivation process requires 1 month and relies on the addition of 2% dimethyl sulfoxide (DMSO) to the culture. Web25 jan. 2024 · The HepaRG cell line is a human bipotent progenitor cell line that can be differentiated into HLCs and biliary epithelial cells [ 19 ]. Whole-genome expression profiling showed that HepaRG cells are remarkably similar to human hepatocyte populations [ 52 ].

Web10 dec. 2024 · Differentiated HepaRG cells show similar or higher gene expression of transporters such as multidrug resistance proteins (MRP1 and MRP2) and organic anion transporting polypeptide (OATP) 2B1 as well as substantial expression of MRP2, MRP3, BSEP, and P-glycoprotein compared to primary human hepatocytes [ 10, 15, 22, 23 ].

Web13 nov. 2002 · To elicit the factors that determine the HBV susceptibility of HepaRG cells, we compared the infection efficiency on poorly and highly differentiated cells. To this purpose, we modulated the differentiation state of HepaRG cells by adding DMSO up to a final concentration of 2% and determined the accumulation of the CYP 3A4 transcripts … essential oils for heart painWeb14 okt. 2024 · Differentiation of HepaRG with 5C or FSK complemented to 1.8% DMSO increase the levels of secreted HBV antigens. ( A ) HepaRG cells were seeded, treated and HBV-infected (500 vge/cells) as indicated. ( B – D ) At the end of the 4 weeks’ differentiation, cells were ( B ) observed by phase contrast microscopy or ( C , D ) … fip inlet vs compressionWeb1 aug. 2024 · Here, we present a novel hepatic maturation medium (HMM) for the differentiation of HepaRG, which is based on a cocktail of soluble molecules that … essential oils for heart problemsWeb9 apr. 2014 · Differentiation of HepaRG Progenitors Into Cholangiocytes HepaRG-HB were treated for 2 days with IL-6 (10 ng/ml), then for 2 days with sodium taurocholate hydrate … essential oils for hecateWeb1 jan. 2010 · The HepaRG cell line, which is a human bipotent progenitor cell line capable to differentiate toward two different cell phenotypes (i.e., biliary-like and hepatocyte-like cells), has been established from a liver tumor associated with chronic hepatitis C. essential oils for heating scentWeb3 mrt. 2024 · The human hepatic cell line HepaRG was obtained from Biopredic International (Rennes, France) and cultured in growth medium consisting of William’s Medium E + GlutaMAX ™ (ThemoFisher Scientific, Landsmeer, The Netherlands) supplemented with 10% fetal bovine serum (FBS; Corning (35-079-CV), United States of … fip in michiganWeb18 jan. 2024 · 1,2-unsaturated Pyrrolizidine Alkaloids (PAs) are secondary plant metabolites that occur as food contaminants. Upon consumption, they can cause severe liver damage. PAs have been shown to induce apoptosis, to have cytotoxic and genotoxic effects, and to impair bile acid homeostasis in the human hepatoma cell line HepaRG. The major mode … essential oils for health