Cyto-id® green detection reagent

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August undefined, 2024

http://biopioneer.com.tw/?p=41098 WebCyto-ID labeling and Flow cytometry: HEK-293 cells were harvested in Accutase solution (Merck Millipore, SF006), rinsed in DPBS and incubated for 30 min at room temperature …

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WebNov 8, 2024 · To determine the effects of LH or AICAR on mean fluorescence intensity of CYTO-ID® Green Detection Reagent, images were converted to maximum intensity projections and processed utilizing ImageJ (National Institutes of Health) analysis software. Web- 2 µL of CYTO-ID® Green Detection Reagent and 1 µL of Hoechst 33342 Nuclear Stain for every 1 mL of 1X Assay Buffer or complete cell growth medium: Downstream tips ; Incase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time. ... highclere medical center

Cyto-ID™ Autophagy Detection Kit

WebEnzo Life Sciences CYTO-ID Autophagy detection kit (1x50tests) A no-transfection, quantitative assay for monitoring autophagy in live cells. CYTO-ID.The probe is a cationic amphiphilic tracer (CAT) dye that rapidly … WebOct 20, 2016 · The Cyto-ID ® Green Reagent is a cationic amphiphilic tracer dye which selectively labels pre-autophagosomes, autophagosomes, and autophagolysosomes. … WebApr 28, 2015 · A. parasiticus SU-1 was grown for 66 h (as described in Figure 1) in the presence of Rapamycin (500 nM, added at 24 h to induce autophagy). 6–8 colonies were stained by Enzo Cyto-ID ® Green Detection Reagent according to manufacturer’s protocol. The stained cells were observed under a fluorescent microscope as described in Methods. how far is walmart from here

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WebDec 1, 2012 · The Cyto-ID green reagent (Cyto-ID) is a. ... Lyso-ID Red Detection Reagent (Lyso-ID) were from Enzo. Life Sciences. Cell culture. Bovine aortic endothelial cells (BAEC) were obtained from. WebThe CYTO-ID® Green Detection Reagent and the Autophagy Inducer (Rapamycin) contain DMSO which is readily absorbed through the skin. DMSO is harmful if ingested or … how far is walnutport pa.from waterbury ctWebThe DYNEX Technologies Automated ELISA System is a cutting edge computer-controlled processing system that achieves full automation of ELISA assays and surrounding … highclere milton keynes

"WebThe CYTO-ID ® Red Tracer Dye fluorescence is independent of pH within normally encountered physiologic ranges and fluorescence intensity per cell is typically unaffected … " - Cyto-id® green detection reagent

Cyto-id® green detection reagent

WebAug 6, 2024 · Autophagy analysis was performed by incubating cells with bafilomycin A1 for 30 min at 37 °C prior to treatment with CYTO-ID Green Detection Reagent, and then analyzing fluorescence by flow cytometry using the … WebThe LYSO-ID ® Green detection kit contains an acidic organelle-selective dye suitable for live-cell staining. Conventional fluorescent stains for acidic organelles, such as Acridine Orange (Prod. No. ENZ-52405), form …

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WebApr 21, 2024 · The CYTO-ID green detection reagent was utilized in this study to confirm the findings of western blotting because it selectively stains the autophagic vesicles. Accumulation of autophagosomes was observed in cells treated with TBs-C and HBSS + chloroquine (CQ) . These findings imply that TBs-C therapy can cause the accumulation … Web1. Product and Company Identification Product Code: 20-2958-xx Product Name: 3'-Amino-Modifier C7 CPG 1000 Product Use: For Research Use Only Manufacturer Information Company Name: Glen Research LLC 22825 Davis Drive Sterling, VA 20164 USA Phone Number: 1-703-437-6191 Web site address: www.glenresearch.com Email address: …

WebFeb 14, 2024 · Cyto-ID green detection reagent, consisting of 1 μL Hoechst 33342, 100 μL Cyto-ID reagent per 1 mL cell culture medium supplemented with 10% FBS was used to stained cells. The plate was stored ... WebThe Cyto-ID™ Autophagy Detection Kit is a member of the CELLestial® product line, reagents and assay kits comprising fluorescent molecular probes that have been …

WebJul 16, 2014 · Cyto-ID Green Detection Reagent Kit (Enzo Life Sciences, Farmingdale, NY) detects autophagosomes and autolysosomes as recommended by manufacturer . In … WebJun 13, 2024 · Inflorescence-based of CYTO-ID® autophagy detection. Primary chondrocytes isolated from the cartilage of WT and GRN −/− mice were treated with DMSO (control) or IL-1β (1 μM) for 24 h, then stained with CYTO-ID® Green Detection Reagent and LysoTracker Red at a concentration of 300 nM was added and incubated at 37°C for …

WebDec 1, 2024 · CYTO-ID® Green dye eliminates background staining of lysosomes seen with other lysosomotrophic dye-based assays that utilize monodansylcadaverine (MDC) (bottom panel). The CYTO-ID® …

WebProduct overview. Autophagy Assay Kit ab139484 measures autophagic vacuoles and monitors autophagic flux in live cells using a dye that selectively labels autophagic … how far is waltham ma from lawrence maWebDec 22, 2024 · The cell residues were washed twice with the assay buffer, and 100 μl of Dual Detection Reagent (2 μl of Cyto-ID Green Detection Reagent and 1 μl of Hoechst 33,342 Nuclear Stain in 1 mL of cell culture medium) was then dispensed in each well. The cells were protected from light and incubated at 37°C for 30 min. highclere nursing home milton keynesWebFluorescent detection, HTS. Contents: CYTO-ID Green Detection Reagent. Hoechst 33342 Nuclear Stain. Autophagy Inducer (Rapamycin). Chloroquine Control. 10X Assay Buffer Catalog No. 50-103-1813 $558.00 / Each Qty Check Availability Add to cart Provide Content Correction highclere oatsWebDec 1, 2024 · The Cyto-ID® Green detection reagent which is a vital coloration of autophagic vacuoles can be read with a FITC filter (Excitation ∼480 nm, Emission ∼530), and the Hoechst 33,342 Nuclear Stain can be read with a DAPI filter set (Excitation ∼340, Emission ∼480). This result was examined using iRiS™ Digital Cell Imaging System … how far is warrenton va from dcWebwere stained with Cyto-ID® Autophagy Green Detection Reagent and Hoechst 33342 nuclear stain (to account for the toxicity of screened compounds and to normalize the green signal). The accumulation of autophagic vesicles was detected using a BioTek Synergy™ Mx multiplate fluorescence reader. Cyto-ID® Autophagy Green fluorescence how far is walvis bay from swakopmundWebIn brief, cells were washed with PCR PBS (supplemented with 5% FBS) and then Gene Primer sequence mixed with Cyto-ID Dual Detection Reagent solution (Cyto-ID Green Detection Reagent and GAPDH Hoechst 33342). highclere on tvWebOct 1, 2024 · The autophagy level of haemocytes was detected with CYTO-ID® Autophagy Detection Kit (Enzo, Switzerland) according to the instruction. ... for 3 h, and then washed two times with M-L15 medium. The haemocytes on the slide were incubated with 200 μL CYTO-ID® Green Detection Reagent (diluted 1:500 in M-L15 medium) at 37 °C in dark … highclere nursing home downs barn