WebApr 4, 2024 · Based on the nature of the antitoxin and the mode of interaction, TA systems have been broadly divided into six types (types I-VI) [3,4]. ... the purified fragments were digested with StuI restriction enzymes and ligated into the pCA24N expression plasmid by ClonExpress TM II One Step Cloning Kit (Vazyme Biotech, Piscataway, NJ, ... WebThe resulting segment was then cloned into pK18mobSacB by a ClonExpress II One Step Cloning Kit (Vazyme, Jiangsu Sheng, China). Full-size DOI: 10.7717/peerj.4253/fig-1 from publication: A ...
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WebAbout Us. Mission: Alleviate Human Suffering from Various Debilitating Diseases - Cell based. therapy and novel drug discovery platform for cancer and metastasis. Founder: … WebMontgomery County, Kansas. / 37.200°N 95.733°W / 37.200; -95.733. / 37.200°N 95.733°W / 37.200; -95.733. Montgomery County (county code MG) is a county … BETTER was first used to diversify the expression of a green fluorescence protein (GFP) reporter in C. glutamicum, a major workhorse in industrial biotechnology used for the production of approximately 70 natural and nonnatural compounds20. The DNA encoding GFP was integrated into the chromosome with a … See more RBS is a G/A-rich nucleotide sequence upstream of the start codon of an mRNA transcript that directly tunes gene expression due to sequence-dependent ribosome recruitment during initiation of protein translation6. … See more Besides RBS, the promoter and the 5′ UTR between RBS and the translational start codon also play important roles in controlling gene … See more We next used BETTER to optimize the pathway for xylose catabolism, which is an industrially important phenotype for lignocellulose … See more To demonstrate an application of BETTER in optimizing more complex metabolic pathways, we conducted decuple-genes regulation to optimize metabolic flux through the 1-deoxy … See more bugs in your ear