WebAug 25, 1992 · Purified CHIP28 from human RBCs was reconstituted into proteoliposomes in order to establish if CHIP28 is itself the functional unit of water channels and to characterize its physiological behavior. CHIP28 proteoliposomes exhibit Pf which is up to 50-fold above that of control liposomes, but permeability to urea and protons is not increased. WebCHIP28 has been proposed to be the major water channel in these and other tissues (Agre et al., 1993). The deduced amino acid sequence of CHIP28 contains 4 cysteines, and the proposed membrane topology includes six probable bilayer-spanning domains (Preston and Agre, 1991). Moreover, the N- and C-terminal halves of the CHIP28 ...

TB3228 Application Note Microchip Technology

WebApr 17, 1992 · The CHIP28 protein is an abundant integral membrane protein in mammalian RBCs and renal proximal tubules and belongs to a family of membrane proteins with unknown functions. Oocytes from Xenopus laevis microinjected with in vitro-transcribed CHIP28 RNA exhibited increased osmotic water permeability; this was reversibly … WebAbbreviations used in this paper: CHIP28, channel-forming integral membrane of 28 kD; gG, a chimpanzee alpha globin domain containing an engineered N-linked glycosylation … campbells beef gravy

The mercury-sensitive residue at cysteine 189 in the CHIP28 …

WebAn abbreviation for CHannel-forming Integral Protein of 28 kDa relative molecular mass. CHIP-28 was purified from the plasma membranes of human erythrocytes and later shown to form channels permeable to water. It was renamed aquaporin1 (AQP1). http://jscholarship.library.jhu.edu/handle/1774.2/34110 WebIn 1993 CHIP28 was renamed aquaporin 1. Looking in retrospect, asking the crucial question, when was the first water channel protein, aquaporin 1, discovered, a fair and clear cut answer would be: the first water channel protein, now called aquaporin 1, was identified or "seen" in situ in the human RBC membrane by Benga and coworkers in 1986. campbell scholtens property consultants